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Метаболічне перепрограмування шляхом біосинтезу ...Frozen liver tissue (~100 mg per sample) was pulverized using the CellCrusher TM cryogenic tissue pulverizer under liquid nitrogen. The soluble polar metabolites were extracted by addition of 1 ml of icecold extraction solvent consisting of 40% acetonitrile, 40% methanol and 20% water, followed by vortexing for 30 sec, and vigorous shaking for ...
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BioSpec Products • Cell Disrupters: A ReviewThere are additional devices used to cryopulverize tissue samples. Caheravirane Schull Co. Cork, Ireland, Spectrum Medical Industries (Carson, CA), and BioSpec Products manufacture freeze fracturing devices called, respectively, CellCrusher, Bessman tissue pulverizer and BioPulverizer.
cTagPAPERCLIP Reveals Alternative Polyadenylation ...Hwang et al. develop cTagPAPERCLIP to profile mRNA 3′ ends in individual cell types from intact tissues in vivo. They use cTagPAPERCLIP to show that mRNA alternative polyadenylation contributes to protein diversity between different cell types and cellular states within the brain.
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cTagPAPERCLIP Reveals Alternative Polyadenylation ...) to profile individual cell types from an intact tissue is to harvest pure cell populations in order to generate the input RNAs. However, the process of tissue dissociation and celltype purifiion, which commonly involves enzymatic digestion and fluorescentactivated cell sorting (FACS), in some cases have been shown to result in cellular stress and alter the gene expression profile
NGlycan Remodeling on Glucagon Receptor Is an Effector of ...06/06/2014 · Frozen liver tissue (80–100 mg) was crushed using the CellCrusher TM cryogenic tissue pulverizer under liquid nitrogen, and 1 ml of icecold solution of (40% acetonitrile, 40% methanol, and 20% water) was added for metabolite extraction. For cells grown in cell culture plates, ...
Spectrum™ Bessman Tissue Pulverizers Large Products ...Consists of a 2 component mortar with handles and a pestle, specifically designed for pulverizing 10 1000 mg of tissue Shop Spectrum™ Bessman Tissue Pulverizers
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(PDF) MicroRNA206: A Potential Circulating Biomarker ...20/02/2014 · Cellcrusher cryogenic tissue pulverizer (Cellcrusher, Cork, Ireland) submerged in liquid nitrogen. The frozen muscle powder was. transferred to a microcentrifuge tube containing ml QIAzol.
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NGlycan Remodeling on Glucagon Receptor Is an Effector of ...Frozen liver tissue (80–100 mg) was crushed using the CellCrusher TM cryogenic tissue pulverizer under liquid nitrogen, and 1 ml of icecold solution of (40% acetonitrile, 40% methanol, and 20% water) was added for metabolite extraction. For cells grown in cell culture plates, metabolites were extracted by adding 1 ml of icecold extraction ...
Metabolische Reprogrammierung durch HexosaminBiosynthese ...Frozen liver tissue (~100 mg per sample) was pulverized using the CellCrusher TM cryogenic tissue pulverizer under liquid nitrogen. The soluble polar metabolites were extracted by addition of 1 ml of icecold extraction solvent consisting of 40% acetonitrile, 40% methanol and 20% water, followed by vortexing for 30 sec, and vigorous shaking for ...
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Biopulverizer™ Soft Tissue Lysing Matrix SetTen tubes of the 5 Lysing Matrix type specific for Soft Tissue samples, perfect for optimization experiments. 2 ml tubes.
tissue grinder pulverizer with liquid nitrogenCellcrusher cryogenic tissue pulverizer, gradient mixer, protein .... Cryogenic tissue pulverizers are used to disrupt frozen tissues with a strong extracellular matrix, ...
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Probing the Hexosamine Biosynthetic Pathway in Human .LCMS/MS Analysis of MetabolitesFrozen liver tissue (80 100 mg) was crushed using the CellCrusher TM cryogenic tissue pulverizer under liquid nitrogen, and 1 ml of icecold solution of (40% ...
SmallAnimal PET of Steroid Hormone Receptors Predicts ...For Western blot analysis, the excised frozen tumors were crushed with a cryogenic tissue pulverizer (Cellcrusher; Ballina). Wholecell lysates were obtained using a 1:1 volume of radioimmunoprecipitation assay buffer (Sigma) containing 2 mM sodium vanadate, protease inhibitor cocktail (1:500, Sigma), and phosphatase inhibitor cocktail (1:100 ...
Smallanimal PET of steroid hormone receptors predicts ...05/06/2012 · For Western blot analysis, the excised frozen tumors were crushed with a cryogenic tissue pulverizer (Cellcrusher; Ballina). Wholecell lysates were obtained using a 1:1 volume of radioimmunoprecipitation assay buffer (Sigma) containing 2 mM sodium vanadate, protease inhibitor cocktail (1:500, Sigma), and phosphatase inhibitor cocktail (1:100 ...
Polygrinder DuallGrinder consists of a conical bottom cylinder with reservoir and a pestle with a conical end. Ideal for grinding and homogenising hard and difficult cells. Pestlecylinder clearance: to mm. Enables a double action: initial grinding by the
